Kalina Peneva, Christop Biskup

Written or an oral examination (100 %), which will be announced at the beginning of the lecture depending on the group size

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H. Zollinger: Color chemistry: Syntheses, properties, and applications of organic dyes and pigments. 3rd ed. Helvetica Chimica Acta 2003.
R.W. Horobin, J.A. Kiernan: Conn's biological stains. 10th ed. Taylor & Francis 2002.
G.T. Hermanson: Bioconjucate techniques. 2nd ed. Acadamic Press 2008.

list of publications given during the lecture

English

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M.Sc. Chemische Biologie: Wahlpflichtmodul

2 SWS Vorlesung, 2 SWS Praktikum

Introduction to pigments, dyes and fluorophores
o Definition, physical and chemical properties of pigments, dyes and fluorophores
o Nomenclature and classification
o Types of dyes applied in biology, fluorescent proteins, quantum dots
o Overview of applications
o Mechanism of biological staining
Reactive staining reagents and fluorescent labels
o Principles and strategies
o Bonding mechanisms to biological materials: functional targets, chemistry of reactive groups Indicators
o Measurement principles of pH-, ion, redox and potential sensitive indicators
o Pitfalls
o Ratiometric measurements
o Nanosensors
Bioconjugate technique

The practical course will encompass synthesis of organic chromophrores like rhodamine dyes, fluorescein, coumarin or others

This course introduces the student to the chemical nature of dyes, the different fluorescent probes and labeling techniques currently applied in advanced fluorescence microscopy imaging methods.The practical course will get the student acquainted with the synthesis methods used to prepared organic chromophores.

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